Production of enzymes from Lichtheimia ramosa using Brazilian savannah fruit wastes as substrate on solid state bioprocessess.
Today we are featuring Electronic Journal of Biotechnology, Vol. 16, No. 5, 2013, which includes "Production of enzymes from Lichtheimia ramosa using Brazilian savannah fruit wastes as substrate on solid state bioprocessess" by de Andrade Silva et al.
The aim of this study is to evaluate the physiology of the fungus Lichtheimia ramosa to determine the microbial growth, and overall production of amylases, B-glucosidases, carboxymethylcellulase, xylanases by means of employing and utilizing waste. The waste is found from the Brazilian savannah fruits bocaiuva, guavira, and the pequi and used as substrate prepared at different temperatures ranging from 25ºC, 30ºC, and 35ºC, during a period of 168 hours. Samples were taken every 24 hours, with results varying.
Overall, the best substrate for B-glucosidases activity was that of the pequi substrate prepared at 30ºC and left for 46 hours. The most suitable substrate for amylase activity was that of bocaiuva substrate prepared at a temperature of 30ºC for 96 hours. Carboxymethylcellulase activity (or CMCase for short)was relatively higher in the guavira substrate after 96 hours at a temperature of 35ºC.
Overall, however, the activity of growth and production that was most expressive came from xylanase in substrate composed of bocaiuva residue after 144 hours, at a prepared temperature of 35ºC. In summary, it can be concluded that the best growth conditions of the L. Ramosa fungus seen in the substrates used, is at a temperature of 35ºC and that the xylanase enzyme has the most potential in solid state bioprocesses.
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The aim of this study is to evaluate the physiology of the fungus Lichtheimia ramosa to determine the microbial growth, and overall production of amylases, B-glucosidases, carboxymethylcellulase, xylanases by means of employing and utilizing waste. The waste is found from the Brazilian savannah fruits bocaiuva, guavira, and the pequi and used as substrate prepared at different temperatures ranging from 25ºC, 30ºC, and 35ºC, during a period of 168 hours. Samples were taken every 24 hours, with results varying.
Overall, the best substrate for B-glucosidases activity was that of the pequi substrate prepared at 30ºC and left for 46 hours. The most suitable substrate for amylase activity was that of bocaiuva substrate prepared at a temperature of 30ºC for 96 hours. Carboxymethylcellulase activity (or CMCase for short)was relatively higher in the guavira substrate after 96 hours at a temperature of 35ºC.
Overall, however, the activity of growth and production that was most expressive came from xylanase in substrate composed of bocaiuva residue after 144 hours, at a prepared temperature of 35ºC. In summary, it can be concluded that the best growth conditions of the L. Ramosa fungus seen in the substrates used, is at a temperature of 35ºC and that the xylanase enzyme has the most potential in solid state bioprocesses.
For this article and others from this issue, click here
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